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Methods in Membrane Lipids

  • Book
  • © 2015

Overview

Editors:
  1. Dylan M. Owen

    1. Department of Physics and Randall Division of Cell and Molecular Biophysics, King’s College London, London, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1232)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to Membrane Lipids

    • Richard M. Epand
    Pages 1-6

  3. Introduction: Membrane Properties (Good) for Life

    • Marek Cebecauer
    Pages 7-17

  4. Introduction to Fluorescence Probing of Biological Membranes

    • Alexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, Yves MĂ©ly
    Pages 19-43

  5. The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs)

    • Charles Cranfield, Sonia Carne, Boris Martinac, Bruce Cornell
    Pages 45-53

  6. Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells

    • Deborah A. Brown
    Pages 55-64

  7. Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning

    • K. R. Levental, I. Levental
    Pages 65-77

  8. Asymmetric Giant Lipid Vesicle Fabrication

    • Peichi C. Hu, Noah Malmstadt
    Pages 79-90

  9. Cholesterol Depletion Using Methyl-β-cyclodextrin

    • Saleemulla Mahammad, Ingela Parmryd
    Pages 91-102

  10. A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems

    • G. Ekin Atilla-Gokcumen, Ulrike S. Eggert
    Pages 103-113

  11. Imaging Membrane Order Using Environmentally Sensitive Fluorophores

    • G. W. Ashdown, Dylan M. Owen
    Pages 115-122

  12. 3D Super-Resolution Imaging by Localization Microscopy

    • Astrid Magenau, Katharina Gaus
    Pages 123-136

  13. Electron Microscopy Methods for Studying Plasma Membranes

    • Alison J. Beckett, Ian A. Prior
    Pages 137-151

  14. Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy

    • Charles Roduit, Giovanni Longo, Giovanni Dietler, Sandor Kasas
    Pages 153-159

  15. Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order

    • Richard K. P. Benninger
    Pages 161-179

  16. Scanning Fluorescence Correlation Spectroscopy on Biomembranes

    • Eduard Hermann, Jonas Ries, Ana J. GarcĂ­a-Sáez
    Pages 181-197

  17. X-Ray Diffraction of Lipid Model Membranes

    • Arwen I. I. Tyler, Robert V. Law, John M. Seddon
    Pages 199-225

  18. Solid State NMR of Lipid Model Membranes

    • Arwen I. I. Tyler, James A. Clarke, John M. Seddon, Robert V. Law
    Pages 227-253

  19. Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis, and Applications

    • Michael Carnell, Alex Macmillan, Renee Whan
    Pages 255-271

  20. The Laurdan Spectral Phasor Method to Explore Membrane Micro-heterogeneity and Lipid Domains in Live Cells

    • Ottavia Golfetto, Elizabeth Hinde, Enrico Gratton
    Pages 273-290
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Keywords

About this book

This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico. Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.

Editors and Affiliations

  • Department of Physics and Randall Division of Cell and Molecular Biophysics, King’s College London, London, United Kingdom

    Dylan M. Owen

Bibliographic Information

  • Book Title: Methods in Membrane Lipids

  • Editors: Dylan M. Owen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1752-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1751-8Published: 21 October 2014

  • Softcover ISBN: 978-1-4939-5399-8Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1752-5Published: 20 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 327

  • Number of Illustrations: 28 b/w illustrations, 15 illustrations in colour

  • Topics: Cell Biology, Membrane Biology, Lipidology

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