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Salmonella

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Heide Schatten

    1. Department of Veterinary Pathobiology, University of Missouri, Columbia, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Abraham Eisenstark

    1. Cancer Research Center, University of Missouri, Columbia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1225)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Luminex® Multiplex Bead Suspension Arrays for the Detection and Serotyping of Salmonella spp.

    • Sherry A. Dunbar, Vivette Brown Ritchie, Michaela R. Hoffmeyer, Gunjot S. Rana, Hongwei Zhang
    Pages 1-27

  3. Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle

    • Dora Kaloyanova, Mijke Vogels, Bas W. M. van Balkom, J. Bernd Helms
    Pages 29-45

  4. Determination of Antimicrobial Resistance in Salmonella spp.

    • Belgode N. Harish, Godfred A. Menezes
    Pages 47-61

  5. Red-Mediated Recombineering of Salmonella enterica Genomes

    • Frederik Czarniak, Michael Hensel
    Pages 63-79

  6. A Method to Introduce an Internal Tag Sequence into a Salmonella Chromosomal Gene

    • Weidong Zhao, Stéphane Méresse
    Pages 81-92

  7. Generation and Use of Site-Directed Chromosomal cyaA′ Translational Fusions in Salmonella enterica

    • Francisco Ramos-Morales, Elena Cardenal-Muñoz, Mar Cordero-Alba, Fernando Baisón-Olmo
    Pages 93-104

  8. Detection of Antimicrobial (Poly)Peptides with Acid Urea Polyacrylamide Gel Electrophoresis Followed by Western Immunoblot

    • Edith Porter, Erika V. Valore, Rabin Anouseyan, Nita H. Salzman
    Pages 105-115

  9. Detecting Non-typhoid Salmonella in Humans by Enzyme-Linked Immunosorbent Assays (ELISAs): Practical and Epidemiological Aspects

    • Katrin G. Kuhn, Hanne-Dorthe Emborg, Karen A Krogfelt, KÃ¥re Mølbak
    Pages 117-126

  10. Study of the Stn Protein in Salmonella; A Regulator of Membrane Composition and Integrity

    • Masayuki Nakano, Eiki Yamasaki, Joel Moss, Toshiya Hirayama, Hisao Kurazono
    Pages 127-138

  11. Development of a Bacterial Nanoparticle Vaccine

    • Carlos Gamazo, Javier Ochoa-Repáraz, Ibai Tamayo, Ana Camacho, Juan M. Irache
    Pages 139-149

  12. Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors

    • Robert Kazmierczak, Elizabeth Choe, Jared Sinclair, Abraham Eisenstark
    Pages 151-163

  13. Applications of Microscopy in Salmonella Research

    • Layla M. Malt, Charlotte A. Perrett, Suzanne Humphrey, Mark A. Jepson
    Pages 165-198

  14. Live Cell Imaging of Intracellular Salmonella enterica

    • Alexander Kehl, Michael Hensel
    Pages 199-225

  15. In Vitro Modeling of Gallbladder-Associated Salmonella spp. Colonization

    • Geoffrey Gonzalez-Escobedo, John S. Gunn
    Pages 227-235

  16. Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

    • Andrea I. Moreno Switt, Alexander Sulakvelidze, Martin Wiedmann, Andrew M. Kropinski, David S. Wishart, Cornelis Poppe et al.
    Pages 237-287

  17. Back Matter

    Pages 289-301
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Keywords

About this book

Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research. With new chapters on molecular assays for detection, identification and serotyping of salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, development of bacterial nanoparticle vaccine, attachment of nanoparticle cargo to biotinylated salmonella for combination bacteriotherapy against tumors, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Salmonella: Methods and Protocols, Second Edition present methods that are of value to investigators in the salmonella field.

Editors and Affiliations

  • Department of Veterinary Pathobiology, University of Missouri, Columbia, USA

    Heide Schatten

  • Cancer Research Center, University of Missouri, Columbia, USA

    Abraham Eisenstark

Bibliographic Information

  • Book Title: Salmonella

  • Book Subtitle: Methods and Protocols

  • Editors: Heide Schatten, Abraham Eisenstark

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1625-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1624-5Published: 25 September 2014

  • Softcover ISBN: 978-1-4939-5440-7Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1625-2Published: 24 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 301

  • Number of Illustrations: 26 b/w illustrations, 26 illustrations in colour

  • Topics: Infectious Diseases, Bacteriology

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