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Protein Design

Methods and Applications

  • Book
  • © 2014

Overview

Editors:
  1. Valentin Köhler

    1. Department of Chemistry, University of Basel, Switzerland

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  • Includes ideas on how to approach a potential protein design project
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1216)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. De Novo Design of Stable α-Helices

    • Alexander Yakimov, Georgy Rychkov, Michael Petukhov
    Pages 1-14

  3. Design of Monomeric Water-Soluble β-Hairpin and β-Sheet Peptides

    • M. Angeles Jiménez
    Pages 15-52

  4. Combination of Theoretical and Experimental Approaches for the Design and Study of Fibril-Forming Peptides

    • Phanourios Tamamis, Emmanouil Kasotakis, Georgios Archontis, Anna Mitraki
    Pages 53-70

  5. Posttranslational Incorporation of Noncanonical Amino Acids in the RNase S System by Semisynthetic Protein Assembly

    • Maika Genz, Norbert Sträter
    Pages 71-87

  6. Design, Synthesis, and Study of Fluorinated Proteins

    • Benjamin C. Buer, E. Neil G. Marsh
    Pages 89-116

  7. High-Quality Combinatorial Protein Libraries Using the Binary Patterning Approach

    • Luke H. Bradley
    Pages 117-128

  8. Methods for Library-Scale Computational Protein Design

    • Lucas B. Johnson, Thaddaus R. Huber, Christopher D. Snow
    Pages 129-159

  9. Symmetric Protein Architecture in Protein Design: Top-Down Symmetric Deconstruction

    • Liam M. Longo, Michael Blaber
    Pages 161-182

  10. Identification of Protein Scaffolds for Enzyme Design Using Scaffold Selection

    • André C. Stiel, Kaspar Feldmeier, Birte Höcker
    Pages 183-196

  11. Computational Design of Novel Enzymes Without Cofactors

    • Matthew D. Smith, Alexandre Zanghellini, Daniela Grabs-Röthlisberger
    Pages 197-210

  12. De Novo Design of Peptide Scaffolds as Novel Preorganized Ligands for Metal-Ion Coordination

    • Aimee J. Gamble, Anna F. A. Peacock
    Pages 211-231

  13. Computational Design of Metalloproteins

    • Avanish S. Parmar, Douglas Pike, Vikas Nanda
    Pages 233-249

  14. Incorporation of Modified and Artificial Cofactors into Naturally Occurring Protein Scaffolds

    • Koji Oohora, Takashi Hayashi
    Pages 251-263

  15. Computational Redesign of Metalloenzymes for Catalyzing New Reactions

    • Per Jr. Greisen, Sagar D. Khare
    Pages 265-273

  16. Back Matter

    Pages 275-279
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Keywords

About this book

Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Thorough and intuitive, Protein Design: Method and Applications, Second Edition provides a number of practical protocols and instructive reviews to aid in the creation of new experiments.

Editors and Affiliations

  • Department of Chemistry, University of Basel, Switzerland

    Valentin Köhler

Bibliographic Information

  • Book Title: Protein Design

  • Book Subtitle: Methods and Applications

  • Editors: Valentin Köhler

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1486-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1485-2Published: 11 September 2014

  • Softcover ISBN: 978-1-4939-4408-8Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1486-9Published: 11 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 279

  • Number of Illustrations: 21 b/w illustrations, 55 illustrations in colour

  • Topics: Protein Science, Computer Appl. in Life Sciences, Protein Structure

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