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Galectins

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Sean R. Stowell

    1. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Richard D. Cummings

    1. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1207)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Evolving Mechanistic Insights into Galectin Functions

    • Connie M. Arthur, Marcelo Dias Baruffi, Richard D. Cummings, Sean R. Stowell
    Pages 1-35

  3. Cloning, Expression, and Purification of Galectins for In Vitro Studies

    • Paul A. Poland, Carol L. Kinlough, Rebecca P. Hughey
    Pages 37-49

  4. Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation

    • Sean R. Stowell, Connie M. Arthur, Richard D. Cummings, Christa L. Feasley
    Pages 51-62

  5. Evaluation of Galectin Binding by Frontal Affinity Chromatography (FAC)

    • Jun Iwaki, Jun Hirabayashi
    Pages 63-74

  6. Probing Lectin–Mucin Interactions by Isothermal Titration Microcalorimetry

    • Tarun K. Dam, C. Fred Brewer
    Pages 75-90

  7. Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis

    • Anne Leppänen, Connie M. Arthur, Sean R. Stowell, Richard D. Cummings
    Pages 91-104

  8. Evaluation of Galectin Binding by Surface Plasmon Resonance

    • Padmaja Mehta-D’souza
    Pages 105-114

  9. Examining Galectin Binding Specificity Using Glycan Microarrays

    • Connie M. Arthur, Lílian Cataldi Rodrigues, Marcelo Dias Baruffi, Harold C. Sullivan, Jamie Heimburg-Molinaro, Dave F. Smith et al.
    Pages 115-131

  10. Methods for Assessing the Effects of Galectins on Leukocyte Trafficking

    • Beatrice R. Gittens, Rachael D. Wright, Dianne Cooper
    Pages 133-151

  11. Examination of the Role of Galectins in Plasma Cell Differentiation

    • Chih-Ming Tsai, Kuo-I Lin
    Pages 153-167

  12. Examination of Galectin-Induced Lattice Formation on Early B-Cell Development

    • Stéphane J. C. Mancini, Latifa Elantak, Annie Boned, Marion Espéli, Françoise Guerlesquin, Claudine Schiff
    Pages 169-184

  13. Detection of Phosphatidylserine Exposure on Leukocytes Following Treatment with Human Galectins

    • Connie M. Arthur, Lilian Cataldi Rodrigues, Marcelo Dias Baruffi, Harold C. Sullivan, Richard D. Cummings, Sean R. Stowell
    Pages 185-200

  14. Examination of Galectins in Phagocytosis

    • Huan-Yuan Chen, I-Chun Weng, Chi-Shan Li, Lei Wan, Fu-Tong Liu
    Pages 201-213

  15. Assessing the Roles of Galectins in Regulating Dendritic Cell Migration Through Extracellular Matrix and Across Lymphatic Endothelial Cells

    • Sandra Thiemann, Jeanette H. Man, Linda G. Baum
    Pages 215-229

  16. Examination of the Role of Galectins in Intestinal Inflammation

    • Atsushi Nishida, Cindy W. Lau, Atsushi Mizoguchi
    Pages 231-248

  17. Study of Galectins in Tumor Immunity: Strategies and Methods

    • Juan P. Cerliani, Tomas Dalotto-Moreno, Daniel Compagno, L. Sebastián Dergan-Dylon, Diego J. Laderach, Lucas Gentilini et al.
    Pages 249-268

  18. Galectins in the Regulation of Platelet Biology

    • Maria A. Romaniuk, Gabriel A. Rabinovich, Mirta Schattner
    Pages 269-283

  19. Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology

    • Iris A. E. Schulkens, Esther A. Kleibeuker, Kitty C. M. Castricum, Arjan W. Griffioen, Victor L. J. L. Thijssen
    Pages 285-291

  20. Regulation of Galectins by Hypoxia and Their Relevance in Angiogenesis: Strategies and Methods

    • Mariana Salatino, Diego O. Croci, Diego J. Laderach, Daniel Compagno, Lucas Gentilini, Tomas Dalotto-Moreno et al.
    Pages 293-304
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Keywords

About this book

Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function.  Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come.

Editors and Affiliations

  • Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, USA

    Sean R. Stowell

  • Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA

    Richard D. Cummings

Bibliographic Information

  • Book Title: Galectins

  • Book Subtitle: Methods and Protocols

  • Editors: Sean R. Stowell, Richard D. Cummings

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1396-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1395-4Published: 25 September 2014

  • Softcover ISBN: 978-1-4939-5296-0Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1396-1Published: 24 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 488

  • Number of Illustrations: 48 b/w illustrations, 52 illustrations in colour

  • Topics: Protein Science

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