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Cytotoxic T-Cells

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Elena Ranieri

    1. Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1186)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii
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  2. Negative and Positive Separation Techniques for the Isolation of Antigen-Specific CD8+ T Cells from Blood and Tumor Tissue

    • Margherita Gigante, Sharon Natasha Cox, Elena Ranieri
    Pages 1-11

  3. In Vitro\Ex Vivo Generation of Cytotoxic T Lymphocytes

    • Margherita Gigante, Elena Ranieri
    Pages 13-20

  4. Phenotypic and Functional Characterization of Cytotoxic T Lymphocytes by Flow Cytometry

    • Iulia Popescu, Matthew Pipeling, Jason Akulian, John McDyer
    Pages 21-47

  5. Intracellular Signaling of CTLs

    • Sharon Natasha Cox
    Pages 49-63

  6. Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR

    • Devin B. Lowe, Jennifer L. Taylor, Walter J. Storkus
    Pages 65-74

  7. CTL ELISPOT Assay

    • Elena Ranieri, Iulia Popescu, Margherita Gigante
    Pages 75-86

  8. Methods of Purification of CTL-Derived Exosomes

    • Angela Montecalvo, Adriana T. Larregina, Adrian E. Morelli
    Pages 87-102

  9. Characterization of CTL by Microscopy

    • Giuseppe Stefano Netti
    Pages 103-119

  10. Intravital Imaging of Cytotoxic T Lymphocytes

    • Gaetano Faleo, Per-Olof Berggren, Antonello Pileggi
    Pages 121-129

  11. The Microarray-Based Approach for the Analysis of the Transcriptome

    • Matteo Accetturo, Paola Pontrelli, Loreto Gesualdo
    Pages 131-199

  12. miRNome Analysis Using Real-Time PCR

    • Paola Pontrelli, Matteo Accetturo, Loreto Gesualdo
    Pages 201-232

  13. Proteomic Approaches by SELDI and MALDI-TOF/MS for CTL Analysis

    • Massimo Papale, Maria Teresa Rocchetti
    Pages 233-242

  14. Two-Dimensional Gel Electrophoresis Approach for CTL Phosphoproteome Analysis

    • Maria Teresa Rocchetti, Massimo Papale, Loreto Gesualdo
    Pages 243-251

  15. Targeting the JAK/STAT Pathway in Cytotoxic T lymphocytes (CTL) by Next Generation Sequencing (NGS)

    • Maddalena Gigante, Sterpeta Diella, Elena Ranieri
    Pages 253-268

  16. Experimental Model for Studying the Involvement of Regulatory Cytotoxic T Cells in Bone Resorption

    • Giacomina Brunetti, Angela Oranger, Silvia Colucci, Maria Grano
    Pages 269-281

  17. CTL and Transplantation: Tissue In Vivo Characterization

    • Federica Rascio, Chiara Divella, Giuseppe Grandaliano
    Pages 283-294

  18. Back Matter

    Pages 295-301
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Keywords

About this book

​ In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology.

Editors and Affiliations

  • Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy

    Elena Ranieri

Bibliographic Information

  • Book Title: Cytotoxic T-Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Elena Ranieri

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1158-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1157-8Published: 23 August 2014

  • Softcover ISBN: 978-1-4939-4686-0Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1158-5Published: 22 August 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 301

  • Number of Illustrations: 6 b/w illustrations, 26 illustrations in colour

  • Topics: Immunology

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