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  • © 2014

Brain Energy Metabolism

  • Includes techniques to investigate brain energy metabolism in differing levels of cell and tissue complexity
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Neuromethods (NM, volume 90)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xiv
  2. Primary Cultures of Astrocytes and Neurons as Model Systems to Study the Metabolism and Metabolite Export from Brain Cells

    • Ketki Tulpule, Michaela C. Hohnholt, Johannes Hirrlinger, Ralf Dringen
    Pages 45-72
  3. Metabolic Mapping of Astrocytes and Neurons in Culture Using Stable Isotopes and Gas Chromatography-Mass Spectrometry (GC-MS)

    • Anne B. Walls, Lasse K. Bak, Ursula Sonnewald, Arne Schousboe, Helle S. Waagepetersen
    Pages 73-105
  4. Metabolic Flux Analysis Tools to Investigate Brain Metabolism In Vitro

    • Ana I. Amaral, Paula M. Alves, Ana P. Teixeira
    Pages 107-144
  5. Fluorescent Nanosensor Based Flux Analysis: Overview and the Example of Glucose

    • L. Felipe Barros, Felipe Baeza-Lehnert, Rocío Valdebenito, Sebastián Ceballo, Karin Alegría
    Pages 145-159
  6. Mitochondrial Bioenergetics Assessed by Functional Fluorescence Dyes

    • Juan Carlos Corona, Michael R. Duchen
    Pages 161-176
  7. RNA Interference as a Tool to Selectively Down-Modulate Protein Function

    • Seila Fernandez-Fernandez, Juan P. Bolaños
    Pages 177-194
  8. Localizing and Quantifying Metabolites In Situ with Luminometry: Induced Metabolic Bioluminescence Imaging (imBI)

    • Stefan Walenta, Nadine F. Voelxen, Ulrike G. A. Sattler, Wolfgang Mueller-Klieser
    Pages 195-216
  9. Measuring Cerebral Hemodynamics and Energy Metabolism by Near-Infrared Spectroscopy

    • Keith St. Lawrence, Kyle Verdecchia, Jonathan Elliott, Mamadou Diop
    Pages 265-292
  10. Compartmental Analysis of Metabolism by 13C Magnetic Resonance Spectroscopy

    • Graeme F. Mason, Lihong Jiang, Kevin L. Behar
    Pages 293-339
  11. Back Matter

    Pages 365-368

About this book

Brain Energy Metabolism addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity. Model systems are discussed, starting from the reductionist approach like primary cell cultures which allow assessing of the properties and functions of a single brain cell type with many different types of analysis, however, at the expense of neglecting the interaction between cell types in the brain. On the other end, analysis in animals and humans in vivo is discussed, maintaining the full complexity of the tissue and the organism but making high demands on the methods of analysis. Written for the popular Neuromethods series, chapters include the kind of detailed description and key implementation advice that aims to support reproducible results in the lab.

Meticulous and authoritative, Brain Energy Metabolism provides an ideal guide for researchers interested in brain energy metabolism with the hope of stimulating more research in this exciting and very important field.

Editors and Affiliations

  • Carl-Ludwig-Institute for Physiology, University of Leipzig, Leipzig, Germany

    Johannes Hirrlinger

  • Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Helle S. Waagepetersen

Bibliographic Information

  • Book Title: Brain Energy Metabolism

  • Editors: Johannes Hirrlinger, Helle S. Waagepetersen

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-1059-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1058-8Published: 16 September 2014

  • Softcover ISBN: 978-1-4939-4466-8Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1059-5Published: 16 September 2014

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIV, 368

  • Number of Illustrations: 45 b/w illustrations, 52 illustrations in colour

  • Topics: Neurosciences, Neurobiology

Buy it now

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 139.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access