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Dengue

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Radhakrishnan Padmanabhan

    1. Georgetown University Medical Center Dept. of Microbiology and Immunology, Washington, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Subhash G. Vasudevan

    1. Duke-NUS GMS Singapore Emerging Infectious Diseases, Singapore, Singapore

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1138)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Modern Quantitative Virological Methods

    1. Front Matter

      Pages 1-1
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    2. Dengue Virus Growth, Purification, and Fluorescent Labeling

      • Summer Zhang, Kuan Rong Chan, Hwee Cheng Tan, Eng Eong Ooi
      Pages 3-14

    3. Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique

      • Milly M. Choy, Duane J. Gubler
      Pages 15-25

    4. Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique

      • Ruklanthi de Alwis, Aravinda M. de Silva
      Pages 27-39

    5. Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy

      • Joanne L. Tan, Shee Mei Lok
      Pages 41-52

    6. Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion

      • Kaiting Ng, John E. Connolly
      Pages 53-74

    7. Pseudo-infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection

      • Swati Mukherjee, Theodore C. Pierson, Kimberly A. Dowd
      Pages 75-97

    8. Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High-Content Imaging

      • Kah Hin Tan, Kitti Chan Wing Ki, Satoru Watanabe, Subhash G. Vasudevan, Manoj Krishnan
      Pages 99-109

  3. Reverse Genetic Systems to Study Virus Replication and Evolution

    1. Front Matter

      Pages 111-111
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    2. Development and Application of Dengue Virus Reverse Genetic Systems

      • Andrew D. Davidson
      Pages 113-130

    3. Construction of Self-Replicating Subgenomic Dengue Virus 4 (DENV4) Replicon

      • Sofia L. Alcaraz-Estrada, Rosa del Angel, Radhakrishnan Padmanabhan
      Pages 131-150

    4. Targeted Mutagenesis of Dengue Virus Type 2 Replicon RNA by Yeast In Vivo Recombination

      • Mark Manzano, Radhakrishnan Padmanabhan
      Pages 151-160

    5. Identification of Dengue-Specific Human Antibody Fragments Using Phage Display

      • Moon Y. F. Tay, Chin Chin Lee, Subhash G. Vasudevan, Nicole J. Moreland
      Pages 161-173

    6. Next-Generation Whole Genome Sequencing of Dengue Virus

      • Pauline Poh Kim Aw, Paola Florez de Sessions, Andreas Wilm, Long Truong Hoang, Niranjan Nagarajan, October M. Sessions et al.
      Pages 175-195

  4. Modern Methods to Study Conformation of RNA Proteins and Their Molecular Interactions

    1. Front Matter

      Pages 197-197
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    2. MPGAfold in Dengue Secondary Structure Prediction

      • Wojciech K. Kasprzak, Bruce A. Shapiro
      Pages 199-224

    3. Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE

      • Joanna Sztuba-Solinska, Stuart F. J. Le Grice
      Pages 225-239

    4. Use of Small-Angle X-ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5

      • Kyung H. Choi, Marc Morais
      Pages 241-252

    5. Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry

      • Alex M. Ward, J. Gunaratne, Mariano A. Garcia-Blanco
      Pages 253-270
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Keywords

About this book

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.

Editors and Affiliations

  • Georgetown University Medical Center Dept. of Microbiology and Immunology, Washington, USA

    Radhakrishnan Padmanabhan

  • Duke-NUS GMS Singapore Emerging Infectious Diseases, Singapore, Singapore

    Subhash G. Vasudevan

Bibliographic Information

  • Book Title: Dengue

  • Book Subtitle: Methods and Protocols

  • Editors: Radhakrishnan Padmanabhan, Subhash G. Vasudevan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0348-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-4939-0347-4Published: 03 April 2014

  • Softcover ISBN: 978-1-4939-4371-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0348-1Published: 02 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 403

  • Number of Illustrations: 33 b/w illustrations, 49 illustrations in colour

  • Topics: Infectious Diseases, Virology, Laboratory Medicine

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