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Current Laboratory Methods in Neuroscience Research

  • Book
  • © 2014

Overview

Editors:
  1. Huangui Xiong

    1. Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Howard E. Gendelman

    1. Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes a variety of laboratory approaches essential for neuroscience research pursuits
  • Covers isolation and culture of brain tissue to animal studies and therapeutic approaches
  • Provides essential and up-to-date technical and methodological guidance for graduate students and investigators

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Neurohistological Techniques

    1. Front Matter

      Pages 1-1
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    2. Brain Tissue Preparation, Sectioning, and Staining

      • Jingdong Zhang, Huangui Xiong
      Pages 3-30

    3. Brain Stereotaxic Injection

      • Jingdong Zhang, Huangui Xiong
      Pages 31-43

    4. Neuronal Tract Tracing with Light and Electron Microscopy

      • Jingdong Zhang, Huangui Xiong
      Pages 45-74

    5. Stereological Analysis

      • Kristi M. Anderson, Adam M. Szlachetka, R. Lee Mosley
      Pages 75-91

  3. In Vitro Preparations

    1. Front Matter

      Pages 93-93
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    2. Preparation and Use of Rodent Hippocampal Slices

      • Huangui Xiong, Jianxun Xia
      Pages 95-103

    3. Single-Cell Neuronal Dissociation for Electrophysiological Studies

      • Yu-Long Li
      Pages 105-113

    4. Isolation and Culture of Human Neurons, Microglia, and Astrocytes

      • Li Wu, Santhi Gorantla
      Pages 115-128

    5. Isolation and Culture of Neural Stem/Progenitor Cells

      • Hui Peng, Qiang Chen, Jialin Zheng
      Pages 129-136

    6. Isolation of Mitochondria from Brain Tissue and Cells

      • Changhai Tian, Jialin Zheng
      Pages 137-143

    7. Isolation of Synaptosomes from Archived Brain Tissues

      • Gurudutt Pendyala, James L. Buescher, Howard S. Fox
      Pages 145-152

    8. Xenopus Oocytes Isolation and Microinjection

      • Huangui Xiong
      Pages 153-161

  4. Leukocyte Isolation and Application in Neuroscience

    1. Front Matter

      Pages 163-163
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    2. Centrifugal Elutriation for Studies of Neuroimmunity

      • Santhi Gorantla, Myhanh Che, Howard E. Gendelman
      Pages 165-175

    3. Flow Cytometry in Neuroscience Research

      • Charles Kuszynski
      Pages 177-184

  5. Standard Laboratory Nucleic Acid and Protein Detections

    1. Front Matter

      Pages 185-185
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    2. Western Blotting Technique in Biomedical Research

      • Jianuo Liu, James Haorah, Huangui Xiong
      Pages 187-200

    3. Polymerase Chain Reaction (PCR) and Real-Time PCR

      • Georgette D. Kanmogne
      Pages 201-210
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Keywords

About this book

Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a “blueprint” for  bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.

Editors and Affiliations

  • Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, USA

    Huangui Xiong, Howard E. Gendelman

Bibliographic Information

  • Book Title: Current Laboratory Methods in Neuroscience Research

  • Editors: Huangui Xiong, Howard E. Gendelman

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-4614-8794-4

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4614-8793-7Published: 05 November 2013

  • Softcover ISBN: 978-1-4939-5216-8Published: 24 August 2016

  • eBook ISBN: 978-1-4614-8794-4Published: 04 November 2013

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XIII, 585

  • Number of Illustrations: 189 b/w illustrations

  • Topics: Neurosciences, Neurology, Neurobiology

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