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Rat Natriuretic Peptide Binds Specifically to Plant Membranes and Induces Stomatal Opening

https://doi.org/10.1006/bbrc.1996.1725Get rights and content

Abstract

Stomatal aperture changes modulate the rate of transpiration and gas exchange in plants. High cellular turgor of the guard cell pair due to water and solute influx leads to stomatal opening, whereas closing is caused by turgor reduction due to water and solute efflux. This process is controlled by plant hormones. We demonstrate that a vertebrate peptide hormone, the rat atrial natriuretic peptide (rANP), induces stomatal opening inTradescantiasp. in a concentration dependent manner and provide evidence that rANP affects the amiloride sensitive Na+/H+channel. Furthermore, rANP was found to bind specifically to plant membranes isolated from leaf tissue. We propose that a plant natriuretic peptide (NP) analogue is part of a multifactorial control system that regulates transpiration and solute movements in plants.

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Cited by (36)

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    Those plant NPs have been shown to participate in a variety of biological processes including stomata movements, ion fluxes and osmoticum-dependent water transport (Maryani et al., 2003). In Tradescantia sp., rat atrial natriuretic peptide (rANP) could specifically bind to plant membranes and induce stomatal opening (Gehring et al., 1996; Gehring and Irving, 2003). Similarly, Hedera helix plant natriuretic peptide could promote stomatal opening in a concentration dependent manner (Billington et al., 1997).

  • The Arabidopsis thaliana natriuretic peptide AtPNP-A is a systemic regulator of leaf dark respiration and signals via the phloem

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    The first evidence for the presence of NP in plants was that radioimmunoassays recognized molecules from extracts of Florida Beauty (Vesely and Giordano, 1991) and that exogenous application of human atrial NP (ANP) can increase plant transpiration and solute flow (Vesely et al., 1993). Subsequently, rat ANP was demonstrated to bind isolated leaf membranes (Gehring et al., 1996; Suwastika et al., 2000) and induce stomatal opening in a concentration, conformation and guanosine 3′,5′-cyclic monophosphate (cGMP) dependent manner (Pharmawati et al., 1998). Plant NPs (PNPs) have since been immunoaffinity purified from a number of different species (Billington et al., 1997; Maryani et al., 2001; Rafudeen et al., 2003).

  • Natriuretic peptides - A class of heterologous molecules in plants

    2003, International Journal of Biochemistry and Cell Biology
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